Modifying Enzymes
Carnabio Usa Inc PIM2/10UG/FULL-LENGTH
PIM2, Full-length, Wild type, Amino Acid 1-311, NP_006866.2, Expressed in Insect (sf21); 10microg
Carnabio Usa Inc BTN - RET / 10UG
BTN-RET, Cytoplasmic domain, Wild type, Amino Acid 658-1114(end), NP_066124.1, 56 kDa, Insect (sf21); 10microg
Ubpbio N-terminally 6xHis-tagged recombinant human UbC13/UEV1A (UbE2N), a ubiquitin conjugating enzyme catalyzing formation of K63 polyubiquitin chains
UbE2N (Ubc13/UEV1A) is a complex that acts as an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Uev1a must interact with Ubc13 because it lacks the active site that other E2s posses. Ube2N has been known to catalyze K63 – linked Ub chains which aides in activation of kinases in NF – kappaB signaling pathway, amongst other tasks.
Medchemexpress LLC Topoisomerase II inhibitor 13 | 451515-89-2 | C22H23N9 | 5 MG
Topoisomerase II inhibitor 13 is a topoisomerase II (Topo II) inhibitor. It demonstrates antiproliferative activity against various cancer cells and induces apoptosis in cancer cells.
- Topoisomerase II (Topo II) inhibitor.
- Shows antiproliferative activity against several cancer cells.
- Induces cancer cell apoptosis.
- Exerts strong inhibitory effect on Topo II activity at 20 μM, and almost completely inhibits Topo II activity at 50 μM.
- Inhibits cancer cell proliferation with varying IC50s across different cell lines (e.g., A549, K562, Raji, HL-60, MDA-MB-231).
LifeSensors USP15 25µg
USP15 (Ubiquitin Specific Protease 15, UNPH4) is a deubiquitinase that associates with the COP9 signalosome (CSN) complex. It regulates various pathways such as the TGF-beta receptor signaling, NFκB and RNF41/NRDP1-PRKN pathways.
AMID BIOSCIENCES Amid Biosciences, T4 RNA Ligase 1, Recombinant, RNA Adapter Ligation, 1000 Units
T4 RNA Ligase 1 is supplied in a 1000 Units format for enzymatic ligation of RNA substrates in molecular biology workflows. The enzyme is commonly used for joining single stranded RNA molecules and attaching adapters during RNA analysis and library preparation method development. Applications include ATP-dependent ligation of single-stranded RNA or DNA, 5 phosphoryl donor to 3 hydroxyl acceptor ligation, RNA adapter ligation, nucleic acid library construction. This product includes 1 mL of 10X reaction buffer, 200 mM Tris-HCl, pH 7.5, 100 mM MgCl2, 20 mM DTT, and 0.2 mL of 10 mM ATP. One unit converts 1 nanomole of 5 phosphorylated 15-mer RNA into phosphatase resistant form in 30 minutes at 37 C. Technical specifications are included below.- Quantity - 1000 Units- Organism - Bacteriophage T4- Activity - 10,000 U/mL- Buffer - 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol- Storage - -20 CThis product is for research use only.
New England Biolabs, Inc. Salt-T4 DNA Ligase – 100000 units
A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher salt concentrations than wild type T4 DNA Ligase. This enzyme will join cohesive end termini at salt concentrations as high as 300 mM without any loss in activity. This enzyme is insensitive to salt carried over from other reaction components (vector or insert DNA) and allows ligation reactions to proceed in alternative reaction buffers with higher levels of salt (e.g., NEBuffer r3.1).
Zymo Research Corporation 5-hmC Glucosyltransferase, 100 U
Glucosylation of 5-hydroxymethylcytosine by 5hmC Glucosyltransferase can be used for sequence specific (see Cat. Nos. D5410 & D5411), locus specific, as well as global quantification of 5- hydroxymethylcytosine.
New England Biolabs, Inc. Sce PUS1 – 5000 pmol
Sce Pseudouridine Synthase I (Sce PUS1) is expressed as a 6X His tagged recombinant protein and converts Uridine to Pseudouridine in RNA. Sce PUS1 has a preference for Uridines in single stranded RNA regions over Uridines in double-stranded RNA. Optimal substrate is an unstructured RNA that is 15 nt long or longer. Sequence-specific pseudouridine modification by Sce PUS1 is an alternative to randomly incorporated modified nucleosides by RNA polymerases.
New England Biolabs, Inc. RNase HII – 1250 units
Ribonuclease HII (RNase HII) is an endoribonuclease that preferentially nicks 5' to a ribonucleotide within the context of a DNA duplex. The enzyme leaves 5' phosphate and 3' hydroxyl ends. RNase HII will also nick at multiple sites along the RNA portion of an Okazaki fragment.
New England Biolabs, Inc. Salt-T4 DNA Ligase – 20000 units
A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher salt concentrations than wild type T4 DNA Ligase. This enzyme will join cohesive end termini at salt concentrations as high as 300 mM without any loss in activity. This enzyme is insensitive to salt carried over from other reaction components (vector or insert DNA) and allows ligation reactions to proceed in alternative reaction buffers with higher levels of salt (e.g., NEBuffer r3.1).
Zymo Research Corporation dsDNA Shearase™ Plus 50 U (1 U/ul) + DCC-5 (D4013) '**'
dsDNA Shearase™ Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5-phosphate and 3-hydroxyl termini. It has a particularly strong preference for double-stranded DNA (dsDNA) and generates random-ended DNA fragments of the desired size in a single step. Sequencing data demonstrates that dsDNA Shearase™ Plus does not introduce any detectable bias in the sequencing library preparation. This enzyme is compatible with low volume inputs thus minimizing sample loss. Digested DNA is easily purified in 10 l with recommended DNA Clean & Concentrator™ technology making it ideal for use in end modification (linker & adapter) procedures and other applications.
New England Biolabs, Inc. β1-3,4 Galactosidase - 400 units
b1-3,4 Galactosidase is an exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing b1-3 and b1-4-linked galactose residues from oligosaccharides.
Bio IVT CYP3A7 Reductase Bactosomes, Human rCYP Enzyme, 1 nmol/vial
CYP3A7 Reductase Bactosomes, Human rCYP Enzyme, 1 nmol/vial